ADP Lumina
Procedures:
Add reagent A and incubate at RT for 30 minutes
Add reagent B and measure luminescence
Alternatively, add “1 solution”, incubate for 30 minutes and measure luminescence
Application:
- Any Kinases
- Ser/Thr or Tyr protein kinases
- Lipid and sugar kinases
- Any ATPases and ion pumps
- Any other enzymes that generate ADP
Features and Benefits:
- Quantify ADP in two easy steps
- No specialized substrate required
- No need for antibodies, radio-isotopes or beads
- Superior sensitivity
- Compatible with all commonly used buffers and reagents
- Tolerates high concentration of ATP, DTT and Hepes
- Luminescence (very low compound interference)
- Homogeneous (ready for 96-, 384-, and 1536- well format)
Standard Curve
Luminescence generated by the assays correlates with ADP concentration.
Application Example 1: Protein Kinase A assayed with ADP Lumina
Application Example 2: PI3 Kinase