ADP Lumina

Procedures:

Add reagent A and incubate at RT for 30 minutes
Add reagent B and measure luminescence

Alternatively, add “1 solution”, incubate for 30 minutes and measure luminescence

Application:

• Any Kinases
  • Ser/Thr or Tyr protein kinases
  • Lipid and sugar kinases
• Any ATPases and ion pumps
• Any other enzymes that generate ADP

Features and Benefits:

• Quantify ADP in two easy steps
• No specialized substrate required
• No need for antibodies, radio-isotopes or beads
• Superior sensitivity
• Compatible with all commonly used buffers and reagents
• Tolerates high concentration of ATP, DTT and Hepes
• Luminescence (very low compound interference)
• Homogeneous (ready for 96-, 384-, and 1536- well format)

Standard Curve

Luminescence generated by the assays correlates with ADP concentration.

Application Example 1: Protein Kinase A assayed with ADP Lumina

Application Example 2: PI3 Kinase